Birgid Schlindwein's
Hypermedia Glossary Of Genetic Terms
Search Results
Birgid Schlindwein'sHypermedia Glossary Of Genetic TermsSearch Results |
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| Chromosome jumping | A technique of isolating clones from a genomic library that are not contiguous by skipping a region between known points on the chromosome. Done usually to bypass regions that are difficult or impossible to walk through or regions known not to be of interest. |
| Clone bank | See genomic library. |
| Genomic library | A collection of clones made from a set of randomly generated overlapping DNA fragments representing the entire genome of an organism. Compare library, arrayed library. |
| Chromosome | The term was proposed by Waldeyer (1888) for the individual threads within a cell nucleus (gk. chroma, colour; soma, body). The self-replicating genetic structures of cells containing the cellular DNA that bears in its nucleotide sequence the linear array of genes. In prokaryotes, chromosomal DNA is circular, and the entire genome is carried on one chromosome. Eukaryotic genomes consist of a number of chromosomes whose DNA is associated with different kinds of proteins. |
| Chromosome walking | Method involving hybridizing a primer of known sequence to a clone from an unordered genomic library and synthesizing a short complementary strand (called walking along a chromosome). The complementary strand is then sequenced and its end used as the next primer for further walking; in this way the adjacent, previously unknown, region is identified and sequenced. The chromosome is thus systematically sequenced from one end to the other. Because primers must be synthesized chemically, a disadvantage of this technique is the large number of different primers needed to walk a long distance. Chromosome walking is also used to locate specific genes by sequencing the chromosomal segments between markers that flank the gene of interest. Cf. chromosome jumping |